GRAP2 antibody (C-Term)

Catalog No. ABIN374093

The Goat Polyclonal anti-GRAP2 antibody has been validated for WB. It is suitable to detect GRAP2 in samples from Human, Mouse and Pig.

Quick Overview for GRAP2 antibody (C-Term) (ABIN374093)

Target: GRAP2 (GRB2-Related Adaptor Protein 2 (GRAP2))

Reactivity: Human, Mouse, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This GRAP2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GRAP2 Antibody

Binding Specificity: C-Term

Specificity: This antibody detects GRAP2 at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Porcine.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Peptide with sequence C-GLFPANYVAPMTR, from the C Terminus of the protein sequence Genename: GRAP2

Application Details

Application Notes: Peptide ELISA: 1/32000. Western blot: 1 - 3 μg/mL. Approx 38 kDa band seen in Jurkat lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for GRAP2

Target: GRAP2 (GRB2-Related Adaptor Protein 2 (GRAP2))

Alternative Name: GRAP2

Background: GRAP2 is a member of the GRB2/Sem5/Drk family. This member is an adaptor-like protein involved in leukocyte-specific protein-tyrosine kinase signaling. Like its related family member, GRB2-related adaptor protein (GRAP), this protein contains an SH2 domain flanked by two SH3 domains. This protein interacts with other proteins, such as GRB2-associated binding protein 1 (GAB1) and the SLP-76 leukocyte protein (LCP2), through its SH3 domains. Transcript variants utilizing alternative polyA sites exist.Synonyms: Adapter protein GRID, GADS, GRB-2-like protein, GRB2-related adapter protein 2, GRB2L, GRBLG, GRBX, GRID, Grf-40, Grf40 adapter protein, Growth factor receptor-binding protein, Hematopoietic cell-associated adapter protein GrpL, SH3-SH2-SH3 adapter Mona

Gene ID: 9402

NCBI Accession: NP_004801

UniProt: O75791

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway